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elNémo has been relocated.
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* TPP_E.coli_OtsB *

B-factor analysis for ID 261012193747778604

Overall B-factor prediction

Correlation= -0.724 for 266 C-alpha atoms.

B-factors for all residues

Residue-by-residue B-factor prediction for CA atoms

B-factors are computed based on the first 100 normal modes (B-PREDICT) and are scaled to match the overall B-factors in the submitted model (B-SCALED). A high correlation with the observations is an indicator that the normal modes capture well the overall flexibility features of the model. Comparison of the observed (B-OBS, blue) and the computed (B-SCALED, red) B-factors for all C-alpha atoms is presented below. Differences between B-factors from normal mode analysis and from X-ray crystallographic data may for example indicate regions where the protein flexibility is modified due to external factors such as tight crystall-packing.

ATOM TYP RES RESID B-PREDICT B-SCALED B-OBS	B-SCALED	B-OBS
2 CA MET 1 0.1867 0.9490 0.6200
10 CA THR 2 0.0574 0.9061 0.7900
17 CA GLU 3 0.0662 0.9090 0.8600
26 CA PRO 4 0.0596 0.9068 0.8800
33 CA LEU 5 0.0428 0.9013 0.8800
41 CA THR 6 0.0672 0.9093 0.8300
48 CA GLU 7 0.0670 0.9093 0.8200
57 CA THR 8 0.0484 0.9031 0.8000
64 CA PRO 9 0.0493 0.9034 0.8000
71 CA GLU 10 0.0699 0.9102 0.8200
80 CA LEU 11 0.0650 0.9086 0.7700
88 CA SER 12 0.1002 0.9203 0.7800
94 CA ALA 13 0.0701 0.9103 0.8400
99 CA LYS 14 0.0583 0.9064 0.9400
108 CA TYR 15 0.0372 0.8994 0.9700
120 CA ALA 16 0.0325 0.8978 0.9800
125 CA TRP 17 0.0235 0.8949 0.9800
139 CA PHE 18 0.0194 0.8935 0.9900
150 CA PHE 19 0.0169 0.8927 0.9900
161 CA ASP 20 0.0162 0.8924 0.9800
169 CA LEU 21 0.0210 0.8940 0.9700
177 CA ASP 22 0.0248 0.8953 0.9500
185 CA GLY 23 0.0265 0.8959 0.9600
189 CA THR 24 0.0216 0.8942 0.9800
196 CA LEU 25 0.0238 0.8949 0.9800
204 CA ALA 26 0.0295 0.8968 0.9800
209 CA GLU 27 0.0464 0.9024 0.9600
218 CA ILE 28 0.0578 0.9062 0.9000
226 CA LYS 29 0.0942 0.9183 0.8900
235 CA PRO 30 0.1612 0.9405 0.8000
242 CA HIS 31 0.1296 0.9300 0.8100
252 CA PRO 32 0.0942 0.9183 0.8000
259 CA ASP 33 0.1212 0.9272 0.8700
267 CA GLN 34 0.1000 0.9202 0.9200
276 CA VAL 35 0.0489 0.9033 0.9500
283 CA VAL 36 0.0495 0.9035 0.9700
290 CA VAL 37 0.0383 0.8998 0.9800
297 CA PRO 38 0.0474 0.9028 0.9800
304 CA ASP 39 0.0617 0.9075 0.9800
312 CA ASN 40 0.0579 0.9063 0.9700
320 CA ILE 41 0.0376 0.8995 0.9800
328 CA LEU 42 0.0383 0.8998 0.9800
336 CA GLN 43 0.0502 0.9037 0.9800
345 CA GLY 44 0.0431 0.9014 0.9700
349 CA LEU 45 0.0338 0.8983 0.9800
357 CA GLN 46 0.0431 0.9014 0.9800
366 CA LEU 47 0.0472 0.9027 0.9600
374 CA LEU 48 0.0389 0.9000 0.9600
382 CA ALA 49 0.0413 0.9008 0.9700
387 CA THR 50 0.0610 0.9073 0.9500
394 CA ALA 51 0.0590 0.9066 0.9400
399 CA SER 52 0.0473 0.9027 0.9500
405 CA ASP 53 0.0627 0.9078 0.9600
413 CA GLY 54 0.0472 0.9027 0.9700
417 CA ALA 55 0.0357 0.8989 0.9800
422 CA LEU 56 0.0278 0.8963 0.9800
430 CA ALA 57 0.0225 0.8945 0.9900
435 CA LEU 58 0.0191 0.8934 0.9900
443 CA ILE 59 0.0168 0.8926 0.9800
451 CA SER 60 0.0169 0.8927 0.9500
457 CA GLY 61 0.0220 0.8944 0.9000
461 CA ARG 62 0.0233 0.8948 0.9100
472 CA SER 63 0.0287 0.8966 0.9100
478 CA MET 64 0.0295 0.8969 0.9400
486 CA VAL 65 0.0483 0.9031 0.9400
493 CA GLU 66 0.0385 0.8998 0.9400
502 CA LEU 67 0.0287 0.8966 0.9700
510 CA ASP 68 0.0370 0.8993 0.9700
518 CA ALA 69 0.0468 0.9026 0.9700
523 CA LEU 70 0.0392 0.9001 0.9800
531 CA ALA 71 0.0375 0.8995 0.9800
536 CA LYS 72 0.0563 0.9057 0.9700
545 CA PRO 73 0.0642 0.9084 0.9700
552 CA TYR 74 0.0513 0.9041 0.9800
564 CA ARG 75 0.0436 0.9015 0.9700
575 CA PHE 76 0.0344 0.8985 0.9800
586 CA PRO 77 0.0296 0.8969 0.9900
593 CA LEU 78 0.0242 0.8951 0.9800
601 CA ALA 79 0.0211 0.8941 0.9700
606 CA GLY 80 0.0213 0.8941 0.9300
610 CA VAL 81 0.0192 0.8934 0.9000
617 CA HIS 82 0.0188 0.8933 0.8600
627 CA GLY 83 0.0220 0.8943 0.9100
631 CA ALA 84 0.0235 0.8949 0.9000
636 CA GLU 85 0.0273 0.8961 0.9300
645 CA ARG 86 0.0273 0.8961 0.9600
656 CA ARG 87 0.0345 0.8985 0.9800
667 CA ASP 88 0.0407 0.9006 0.9700
675 CA ILE 89 0.0493 0.9034 0.9600
683 CA ASN 90 0.0782 0.9130 0.9500
691 CA GLY 91 0.0660 0.9090 0.9500
695 CA LYS 92 0.0696 0.9102 0.9700
704 CA THR 93 0.0507 0.9039 0.9600
711 CA HIS 94 0.0497 0.9036 0.9300
721 CA ILE 95 0.0489 0.9033 0.9100
729 CA VAL 96 0.0469 0.9026 0.8700
736 CA HIS 97 0.0758 0.9122 0.8300
746 CA LEU 98 0.0638 0.9082 0.8300
754 CA PRO 99 0.1065 0.9224 0.8700
761 CA ASP 100 0.1195 0.9267 0.8300
769 CA ALA 101 0.1037 0.9215 0.8900
774 CA ILE 102 0.0647 0.9085 0.9100
782 CA ALA 103 0.0547 0.9052 0.9000
787 CA ARG 104 0.0852 0.9153 0.9200
798 CA ASP 105 0.0768 0.9125 0.9400
806 CA ILE 106 0.0473 0.9028 0.9500
814 CA SER 107 0.0571 0.9060 0.9400
820 CA VAL 108 0.0876 0.9161 0.9400
827 CA GLN 109 0.0720 0.9109 0.9400
836 CA LEU 110 0.0546 0.9052 0.9600
844 CA HIS 111 0.0860 0.9156 0.9400
854 CA THR 112 0.1189 0.9265 0.9300
861 CA VAL 113 0.0966 0.9191 0.9400
868 CA ILE 114 0.0858 0.9155 0.9400
876 CA ALA 115 0.1528 0.9377 0.9300
881 CA GLN 116 0.1629 0.9411 0.9500
890 CA TYR 117 0.1263 0.9289 0.9600
902 CA PRO 118 0.1683 0.9429 0.9500
909 CA GLY 119 0.1496 0.9367 0.9400
913 CA ALA 120 0.0961 0.9189 0.9600
918 CA GLU 121 0.0802 0.9136 0.9500
927 CA LEU 122 0.0552 0.9054 0.9500
935 CA GLU 123 0.0510 0.9040 0.9000
944 CA THR 124 0.0430 0.9013 0.8800
951 CA LYS 125 0.0370 0.8993 0.7900
960 CA GLY 126 0.0456 0.9022 0.8100
964 CA MET 127 0.0279 0.8963 0.8500
972 CA ALA 128 0.0189 0.8933 0.8800
977 CA PHE 129 0.0220 0.8944 0.9200
988 CA ALA 130 0.0270 0.8960 0.9200
993 CA LEU 131 0.0371 0.8994 0.9500
1001 CA HIS 132 0.0582 0.9064 0.9400
1011 CA TYR 133 0.0843 0.9150 0.9500
1023 CA ARG 134 0.1234 0.9280 0.9200
1034 CA GLN 135 0.1697 0.9433 0.9100
1043 CA ALA 136 0.1423 0.9342 0.9400
1048 CA PRO 137 0.1415 0.9340 0.9500
1055 CA GLN 138 0.1688 0.9430 0.9700
1064 CA HIS 139 0.1131 0.9246 0.9700
1074 CA GLU 140 0.0917 0.9175 0.9600
1083 CA ASP 141 0.1191 0.9266 0.9700
1091 CA ALA 142 0.1035 0.9214 0.9700
1096 CA LEU 143 0.0589 0.9066 0.9700
1104 CA MET 144 0.0573 0.9061 0.9700
1112 CA THR 145 0.0808 0.9139 0.9700
1119 CA LEU 146 0.0602 0.9070 0.9600
1127 CA ALA 147 0.0380 0.8997 0.9600
1132 CA GLN 148 0.0590 0.9066 0.9700
1141 CA ARG 149 0.0752 0.9120 0.9600
1152 CA ILE 150 0.0487 0.9032 0.9500
1160 CA THR 151 0.0473 0.9027 0.9500
1167 CA GLN 152 0.0831 0.9146 0.9600
1176 CA ILE 153 0.0819 0.9142 0.9500
1184 CA TRP 154 0.0631 0.9080 0.9400
1198 CA PRO 155 0.0717 0.9108 0.9300
1205 CA GLN 156 0.0572 0.9060 0.9300
1214 CA MET 157 0.0321 0.8977 0.9400
1222 CA ALA 158 0.0325 0.8978 0.9300
1227 CA LEU 159 0.0273 0.8961 0.9200
1235 CA GLN 160 0.0249 0.8953 0.8800
1244 CA GLN 161 0.0431 0.9013 0.8800
1253 CA GLY 162 0.0553 0.9054 0.8300
1257 CA LYS 163 0.1215 0.9273 0.8100
1266 CA CYS 164 0.0854 0.9154 0.9000
1272 CA VAL 165 0.0476 0.9028 0.9100
1279 CA VAL 166 0.0312 0.8974 0.9400
1286 CA GLU 167 0.0176 0.8929 0.9200
1295 CA ILE 168 0.0164 0.8925 0.9400
1303 CA LYS 169 0.0164 0.8925 0.9000
1312 CA PRO 170 0.0230 0.8947 0.9100
1319 CA ARG 171 0.0362 0.8991 0.9100
1330 CA GLY 172 0.0513 0.9041 0.8900
1334 CA THR 173 0.0219 0.8943 0.9100
1341 CA SER 174 0.0209 0.8940 0.9300
1347 CA LYS 175 0.0186 0.8932 0.9500
1356 CA GLY 176 0.0222 0.8944 0.9700
1360 CA GLU 177 0.0259 0.8957 0.9600
1369 CA ALA 178 0.0225 0.8945 0.9700
1374 CA ILE 179 0.0226 0.8946 0.9800
1382 CA ALA 180 0.0298 0.8969 0.9800
1387 CA ALA 181 0.0348 0.8986 0.9700
1392 CA PHE 182 0.0312 0.8974 0.9800
1403 CA MET 183 0.0383 0.8998 0.9800
1411 CA GLN 184 0.0586 0.9065 0.9700
1420 CA GLU 185 0.0514 0.9041 0.9700
1429 CA ALA 186 0.0590 0.9066 0.9700
1434 CA PRO 187 0.0518 0.9042 0.9700
1441 CA PHE 188 0.0410 0.9007 0.9800
1452 CA ILE 189 0.0610 0.9073 0.9700
1460 CA GLY 190 0.0745 0.9118 0.9700
1464 CA ARG 191 0.0468 0.9026 0.9800
1475 CA THR 192 0.0414 0.9008 0.9800
1482 CA PRO 193 0.0286 0.8965 0.9800
1489 CA VAL 194 0.0237 0.8949 0.9800
1496 CA PHE 195 0.0177 0.8929 0.9900
1507 CA LEU 196 0.0166 0.8926 0.9900
1515 CA GLY 197 0.0176 0.8929 0.9800
1519 CA ASP 198 0.0216 0.8942 0.9600
1527 CA ASP 199 0.0259 0.8956 0.9200
1535 CA LEU 200 0.0256 0.8956 0.9000
1543 CA THR 201 0.0209 0.8940 0.8900
1550 CA ASP 202 0.0184 0.8932 0.9600
1558 CA GLU 203 0.0240 0.8950 0.9600
1567 CA SER 204 0.0250 0.8954 0.9600
1573 CA GLY 205 0.0216 0.8942 0.9800
1577 CA PHE 206 0.0224 0.8945 0.9900
1588 CA ALA 207 0.0337 0.8982 0.9800
1593 CA ALA 208 0.0330 0.8980 0.9800
1598 CA VAL 209 0.0281 0.8964 0.9900
1605 CA ASN 210 0.0367 0.8992 0.9800
1613 CA ARG 211 0.0608 0.9072 0.9800
1624 CA LEU 212 0.0558 0.9056 0.9800
1632 CA GLY 213 0.0574 0.9061 0.9600
1636 CA GLY 214 0.0360 0.8990 0.9700
1640 CA MET 215 0.0274 0.8961 0.9600
1648 CA SER 216 0.0235 0.8949 0.9800
1654 CA VAL 217 0.0224 0.8945 0.9800
1661 CA LYS 218 0.0230 0.8947 0.9800
1670 CA ILE 219 0.0253 0.8955 0.9800
1678 CA GLY 220 0.0370 0.8993 0.9600
1682 CA THR 221 0.0913 0.9173 0.9300
1689 CA GLY 222 0.1198 0.9268 0.9200
1693 CA ALA 223 0.0952 0.9186 0.9400
1698 CA THR 224 0.0430 0.9013 0.9700
1705 CA GLN 225 0.0353 0.8988 0.9700
1714 CA ALA 226 0.0292 0.8967 0.9800
1719 CA SER 227 0.0376 0.8995 0.9700
1725 CA TRP 228 0.0337 0.8982 0.9700
1739 CA ARG 229 0.0316 0.8976 0.9800
1750 CA LEU 230 0.0326 0.8979 0.9800
1758 CA ALA 231 0.0460 0.9023 0.9700
1763 CA GLY 232 0.0388 0.8999 0.9700
1767 CA VAL 233 0.0324 0.8978 0.9800
1774 CA PRO 234 0.0416 0.9009 0.9800
1781 CA ASP 235 0.0375 0.8995 0.9800
1789 CA VAL 236 0.0281 0.8964 0.9800
1796 CA TRP 237 0.0340 0.8983 0.9800
1810 CA SER 238 0.0422 0.9011 0.9700
1816 CA TRP 239 0.0343 0.8984 0.9600
1830 CA LEU 240 0.0329 0.8980 0.9600
1838 CA GLU 241 0.0441 0.9017 0.9600
1847 CA MET 242 0.0484 0.9031 0.9400
1855 CA ILE 243 0.0428 0.9012 0.9400
1863 CA THR 244 0.0476 0.9028 0.9300
1870 CA THR 245 0.0736 0.9115 0.9300
1877 CA ALA 246 0.0634 0.9081 0.9200
1882 CA LEU 247 0.0527 0.9045 0.9000
1890 CA GLN 248 0.0681 0.9096 0.8900
1899 CA GLN 249 0.0858 0.9155 0.8900
1908 CA LYS 250 0.0837 0.9148 0.8600
1917 CA ARG 251 0.0950 0.9186 0.8500
1928 CA GLU 252 0.0932 0.9179 0.8400
1937 CA ASN 253 0.1277 0.9294 0.8300
1945 CA ASN 254 0.1620 0.9408 0.8000
1953 CA ARG 255 0.1749 0.9450 0.7600
1964 CA SER 256 0.2065 0.9555 0.7200
1970 CA ASP 257 0.3201 0.9932 0.6600
1978 CA ASP 258 0.4225 1.0271 0.6000
1986 CA TYR 259 0.4802 1.0463 0.5500
1998 CA GLU 260 0.6310 1.0963 0.5700
2007 CA SER 261 0.8929 1.1831 0.5700
2013 CA PHE 262 1.0894 1.2482 0.5400
2024 CA SER 263 1.2596 1.3046 0.5400
2030 CA ARG 264 1.7017 1.4512 0.4900
2041 CA SER 265 2.3114 1.6533 0.4800
2047 CA ILE 266 3.1017 1.9153 0.4700

If you find results from this site helpful for your research, please cite one of our papers:

K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004.
K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography.

elNémo is maintained by Yves-Henri Sanejouand.
It was developed by Karsten Suhre.
Between 2003 and 2014, it was hosted by IGS (Marseille).
Between 2015 and 2025, it was hosted by US2B (Nantes).
Last modification: april 24th, 2026.

Should you encounter any unexpected behaviour, please let us know. elNémo has been relocated. **Some cleaning from time to time** Sorry for the inconvenience.